Multi-kernel deconvolution applied to confocal fluorescence microscopy with engineered point spread function

Multi-kernel deconvolution applied to confocal fluorescence microscopy with engineered point spread function

Point spread function engineering in confocal scanning microscopy

Confocal scanning microscopes are imaging systems that are mainly featured by their unique depth-discrimination capacity when imaging three-dimensional objects. Along the past few years, our research group has done several attempts to improve their axial resolution by means of the so-called point-spread-function (PSF) engineering method. That is, by designing diffractive elements that properly ...

Enhancement of Point Spread Function of Planar Fluorescent Imaging System Using Iterative Deconvolution Method

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Fixed Vs Adaptable Point Spread Function (psf) in Deconvolution Two-photon Microscopy of Pathological Neo-vasculature

Pathological neo-vascularization, i.e., the expansion of the vascular bed in solid tumors and after hindlimb ischemia and myocardial infarction, occurs through angiogenesis and/or arteriogenesis [1]. Quantification of microvascular morphology would allow evaluation of the effect of proand anti-angiogenic therapy. However, due to the point spread function (PSF) of the TPLSM setup, quantification...

Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function.

We present a technique to systematically measure the change in the blurring function of an optical microscope with distance between the source and the coverglass (the depth) and demonstrate its utility in three-dimensional (3D) deconvolution. By controlling the axial positions of the microscope stage and an optically trapped bead independently, we can record the 3D blurring function at differen...